Mycobacterium Tuberculosis DNA PCR Trunat

• Test Name: Mycobacterium Tuberculosis DNA PCR Trunat Test
• Test Type: Molecular diagnostic test
• Specimen Required: Sputum, bronchoalveolar lavage (BAL), tissue biopsy, or other clinical specimens
• Methodology: Polymerase Chain Reaction (PCR) using the Truenat platform
• Purpose: Detects the presence of Mycobacterium tuberculosis DNA to diagnose tuberculosis (TB) and identify drug-resistant strains.

• Diagnosis: Rapid diagnosis of active tuberculosis infection in suspected patients.
• Screening: Used for early detection of TB, especially in high-risk populations.
• Drug Resistance: Detects mutations associated with resistance to first-line TB drugs, aiding in appropriate treatment decisions.
• Monitoring: Helps monitor the effectiveness of TB treatment by detecting the presence of Mycobacterium tuberculosis DNA over time.

• Components:
  • Test cartridge with reagents for DNA extraction, amplification, and detection.
  • Portable Truenat device for automated processing and real-time PCR analysis.
  • Sample collection kit with instructions for proper specimen handling.
• Procedure:
  • Collection of a clinical specimen such as sputum or tissue.
  • The sample is loaded into the Truenat device, which processes the specimen for DNA extraction and amplification.
  • Real-time PCR is performed to detect Mycobacterium tuberculosis DNA, with results available within a short time.

• Positive Result: Indicates the presence of Mycobacterium tuberculosis DNA, confirming an active TB infection.
• Negative Result: Suggests the absence of Mycobacterium tuberculosis DNA, but clinical correlation and further testing may be required if TB is still suspected.
• Drug Resistance Detection: Identifies specific mutations associated with resistance to TB drugs, guiding personalized treatment plans.
• Clinical Correlation: Results should be interpreted alongside clinical symptoms, patient history, and other diagnostic tests.

• Qualitative: Provides a positive or negative result for the presence of Mycobacterium tuberculosis DNA.
• Drug Resistance Markers: This may include information on resistance to drugs such as rifampicin.
• Turnaround Time: Typically within 2 hours, depending on the sample type and workflow.

Q. What is the Mycobacterium Tuberculosis DNA PCR Trunat Test?
The Mycobacterium Tuberculosis DNA PCR Trunat Test is a rapid molecular diagnostic test that detects the presence of Mycobacterium tuberculosis DNA in clinical samples, confirming the diagnosis of tuberculosis
 

Q. Why is the Mycobacterium Tuberculosis DNA PCR Trunat Test ordered?
This test is ordered to quickly diagnose active tuberculosis, screen high-risk populations, and detect drug-resistant strains of Mycobacterium tuberculosis
 

Q. How is the Mycobacterium Tuberculosis DNA PCR Trunat Test performed?
A clinical specimen like sputum is collected, and the sample is processed using the Truenat device, which performs PCR to detect the DNA of Mycobacterium tuberculosis
 

Q. What do the results of the Mycobacterium Tuberculosis DNA PCR Trunat Test indicate?
A positive result confirms an active TB infection, while a negative result suggests the absence of the bacteria, though additional tests may be needed if symptoms persist
 

Q. Do I need to prepare for the Mycobacterium Tuberculosis DNA PCR Trunat Test?
No special preparation is needed for the test, but it is important to provide a good quality specimen, such as an early morning sputum sample
 

Q. How long does it take to receive results from the Mycobacterium Tuberculosis DNA PCR Trunat Test?
Results are typically available within 2 hours after the sample is processed, making it a rapid diagnostic tool for TB
 

Q. What should I do if my Mycobacterium Tuberculosis DNA PCR Trunat Test results are positive?
If your test results are positive, your healthcare provider will discuss treatment options with you, including the possibility of drug-resistant TB and the need for specific therapies